Sequence-specific Nucleic Acid Detection from Binary Pore Conductance Measurement
نویسندگان
چکیده
منابع مشابه
Sequence-specific nucleic acid detection from binary pore conductance measurement.
We describe a platform for sequence-specific nucleic acid (NA) detection utilizing a micropipet tapered to a 2 μm diameter pore and 3 μm diameter polystyrene beads to which uncharged peptide nucleic acid (PNA) probe molecules have been conjugated. As the target NAs hybridize to the complementary PNA-beads, the beads acquire negative charge and become electrophoretically mobile. An applied elect...
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Inhibition of gene expression is of great interest to explore gene function or for therapeutic applications. Oligonucleotides that interact with a specific RNA (antisense strategy) or DNA (antigene strategy) sequence are very attractive to disrupt gene expression. Structural modifications brought to the last generation of oligonucleotides like PNAs (peptide nucleic acids) have led to molecules ...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2012
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja3059205